Publikationen Tissue Engineering



Aper, T., Wilhelmi, M., Gebhardt, C., Hoeffler, K., Benecke, N., Hilfiker, A., and Haverich, A. 2016. Novel method for the generation of tissue-engineered vascular grafts based on a highly compacted fibrin matrix. Acta Biomater 29:21


Braniste, T., Tiginyanu, I., Horvath, T., Raevschi, S., Cebotari, S., Lux, M., Haverich, A., and Hilfiker, A. 2016. Viability and proliferation of endothelial cells upon exposure to GaN nanoparticles. Beilstein J Nanotechnol 7:1330


Kuroda, M. I., Hilfiker, A., and Lucchesi, J. C. 2016. Dosage Compensation in Drosophila-a Model for the Coordinate Regulation of Transcription. Genetics 204, no. 2:435


Lux, M., Andree, B., Horvath, T., Nosko, A., Manikowski, D., Hilfiker-Kleiner, D., Haverich, A., and Hilfiker, A. 2016. In vitro maturation of large-scale cardiac patches based on a perfusable starter matrix by cyclic mechanical stimulation. Acta Biomater 30:177


Ramm, R., Niemann, H., Petersen, B., Haverich, A., and Hilfiker, A. 2016. Decellularized GGTA1-KO pig heart valves do not bind preformed human xenoantibodies. Basic Res Cardiol 111, no. 4:39


Theodoridis, K., Muller, J., Ramm, R., Findeisen, K., Andree, B., Korossis, S., Haverich, A., and Hilfiker, A. 2016. Effects of combined cryopreservation and decellularization on the biomechanical, structural and biochemical properties of porcine pulmonary heart valves. Acta Biomater 43:71


Tudorache, I., Theodoridis, K., Baraki, H., Sarikouch, S., Bara, C., Meyer, T., Hoffler, K., Hartung, D., Hilfiker, A., Haverich, A., and Cebotari, S. 2016. Decellularized aortic allografts versus pulmonary autografts for aortic valve replacement in the growing sheep model: haemodynamic and morphological results at 20 months after implantation. Eur J Cardiothorac Surg 49, no. 4:1228





Goecke, T., Hilfiker, A., Haverich, A., and Cebotari, S. 2015. Investigation of inflammatory response of decellularized porcine aortic tissue in mice: can we rely on this experimental setting? Eur J Cardiothorac Surg 47, no. 3:e90


Goecke, T., Tudorache, I., Hilfiker, A., Haverich, A., and Cebotari, S. 2015. [Prefabrication of heart valves]. Chirurg 86, no. 3:251


Hofmann, A. D., Hilfiker, A., Haverich, A., Andree, B., Kuebler, J., and Ure, B. 2015. BioVaM in the rat model: a new approach of vascularized 3D tissue for esophageal replacement. Eur J Pediatr Surg 25, no. 2:181


Iablonskii, P., Cebotari, S., Tudorache, I., Granados, M., Morticelli, L., Goecke, T., Klein, N., Korossis, S., Hilfiker, A., and Haverich, A. 2015. Tissue-engineered mitral valve: morphology and biomechanics dagger. Interact Cardiovasc Thorac Surg 20, no. 6:712


Ramm, R., Hartmann, T., Tudorache, I., Haverich, A., and Hilfiker, A. 2015. No evidence for alphaGal epitope transfer from media containing FCS onto human endothelial cells in culture. Xenotransplantation 22, no. 5:345


Theodoridis, K., Tudorache, I., Calistru, A., Cebotari, S., Meyer, T., Sarikouch, S., Bara, C., Brehm, R., Haverich, A., and Hilfiker, A. 2015. Successful matrix guided tissue regeneration of decellularized pulmonary heart valve allografts in elderly sheep. Biomaterials 52:221


Wang, S., Oldenhof, H., Goecke, T., Ramm, R., Harder, M., Haverich, A., Hilfiker, A., and Wolkers, W. F. 2015. Sucrose Diffusion in Decellularized Heart Valves for Freeze-Drying. Tissue Eng Part C Methods 21, no. 9:922


Wolkers, W. F., and Hilfiker, A. 2015. Freeze-drying of decellularized heart valve tissues. Methods Mol Biol 1257:499





Andree, B., Bela, K., Horvath, T., Lux, M., Ramm, R., Venturini, L., Ciubotaru, A., Zweigerdt, R., Haverich, A., and Hilfiker, A. 2014. Successful re-endothelialization of a perfusable biological vascularized matrix (BioVaM) for the generation of 3D artificial cardiac tissue. Basic Res Cardiol 109, no. 6:441


Eberhard, J., Stiesch, M., Kerling, A., Bara, C., Eulert, C., Hilfiker-Kleiner, D., Hilfiker, A., Budde, E., Bauersachs, J., Kuck, M., Haverich, A., Melk, A., and Tegtbur, U. 2014. Moderate and severe periodontitis are independent risk factors associated with low cardiorespiratory fitness in sedentary non-smoking men aged between 45 and 65 years. J Clin Periodontol 41, no. 1:31


Hochberger, J., Koehler, P., Wedi, E., Gluer, S., Rothstein, R. I., Niemann, H., Hilfiker, A., Gonzalez, S., and Kruse, E. 2014. Transplantation of mucosa from stomach to esophagus to prevent stricture after circumferential endoscopic submucosal dissection of early squamous cell. Gastroenterology 146, no. 4:906


Melk, A., Tegtbur, U., Hilfiker-Kleiner, D., Eberhard, J., Saretzki, G., Eulert, C., Kerling, A., Nelius, A. K., Homme, M., Strunk, D., Berliner, D., Rontgen, P., Kuck, M., Bauersachs, J., Hilfiker, A., Haverich, A., Bara, C., and Stiesch, M. 2014. Improvement of biological age by physical activity. Int J Cardiol 176, no. 3:1187


Minol, J. P., Baraki, H., Akhyari, P., Bagaev, E., Suprunov, M., Brandes, G., Bara, C., Hort, W., Hurschler, C., Sigler, M., Haverich, A., Hilfiker, A., and Lichtenberg, A. 2014. Tracheal cartilage - evaluating the potential of a novel biomaterial for reconstructive cardiovascular procedures. J Tissue Eng Regen Med 8, no. 11:850


Vukadinovic-Nikolic, Z., Andree, B., Dorfman, S. E., Pflaum, M., Horvath, T., Lux, M., Venturini, L., Bar, A., Kensah, G., Lara, A. R., Tudorache, I., Cebotari, S., Hilfiker-Kleiner, D., Haverich, A., and Hilfiker, A. 2014. Generation of bioartificial heart tissue by combining a three-dimensional gel-based cardiac construct with decellularized small intestinal submucosa. Tissue Eng Part A 20, no. 3-4:799


Wang, S., Oldenhof, H., Dai, X., Haverich, A., Hilfiker, A., Harder, M., and Wolkers, W. F. 2014. Protein stability in stored decellularized heart valve scaffolds and diffusion kinetics of protective molecules. Biochim Biophys Acta 1844, no. 2:430




Andree, B., Bar, A., Haverich, A., and Hilfiker, A. 2013. Small intestinal submucosa segments as matrix for tissue engineering: review. Tissue Eng Part B Rev19, no. 4:279.


Aper, T., Teebken, O.E., Kruger, A., Heisterkamp, A., Hilfiker, A., and Haverich, A. 2013. Development of implantable autologous small-calibre vascular grafts from peripheral blood samples. Zentralbl Chir 138, no. 2:173.


Ciubotaru, A., Cebotari, S., Tudorache, I., Beckmann, E., Hilfiker, A., and Haverich, A. 2013. Biological heart valves. Biomed Tech (Berl) 58, no. 5:389.


Derks, M., Sturm, T., Haverich, A., and Hilfiker, A. 2013. Isolation and chondrogenic differentiation of porcine perichondrial progenitor cells for the purpose of cartilage tissue engineering. Cells Tissues Organs 198, no. 3:179


Schilling, T., Brandes, G., Tudorache, I., Cebotari, S., Hilfiker, A., Meyer, T., Biskup, C., Bauer, M., Waldmann, K.H., Bach, F.W., Haverich, A., and Hassel, T. 2013. In vivo degradation of magnesium alloy LA63 scaffolds for temporary stabilization of biological myocardial grafts in a swine model. Biomed Tech (Berl) 58, no. 5:407.


Steele, T.W., Huang, C.L., Nguyen, E., Sarig, U., Kumar, S., Widjaja, E., Loo, J.S., Machluf, M., Boey, F., Vukadinovic, Z., Hilfiker, A., and Venkatraman, S.S. 2013. Collagen-cellulose composite thin films that mimic soft-tissue and allow stem-cell orientation. J Mater Sci Mater Med 24, no. 8:2013.


Tudorache, I., Calistru, A., Baraki, H., Meyer, T., Hoffler, K., Sarikouch, S., Bara, C., Gorler, A., Hartung, D., Hilfiker, A., Haverich, A., and Cebotari, S. 2013. Orthotopic replacement of aortic heart valves with tissue-engineered grafts. Tissue Eng Part A 19, no. 15-16:1686.


Westhoff-Bleck, M., Podewski, E., Hilfiker, A., and Hilfiker-Kleiner, D. 2013. Cardiovascular disorders in pregnancy: diagnosis and management. Best Pract Res Clin Obstet Gynaecol 27, no. 6:821.



Wang, S., Goecke, T., Meixner, C., Haverich, A., Hilfiker, A., and Wolkers, W. F. 2012. Freeze-dried heart valve scaffolds. Tissue Eng Part C Methods 18, no. 7:517




Hilfiker A, Kasper C, Hass R, Haverich A: Mesenchymal stem cells and progenitor cells in connective tissue engineering and regenerative medicine: is there a future for transplantation? Langenbecks Arch Surg 2011; 396, no. 4:489-97.
Hoch M, Fischer P, Stapel B, Missol-Kolka E, Sekkali B, Scherr M, Favret F, Braun T, Eder M, Schuster-Gossler K, Gossler A, Hilfiker A, Balligand J L, Drexler H, Hilfiker-Kleiner D: Erythropoietin Preserves the Endothelial Differentiation Capacity of Cardiac Progenitor Cells and Reduces Heart Failure during Anticancer Therapies. Cell Stem Cell 2011; 9, no. 2:131.




Bär A, Dorfman SE, Fischer P, Hilfiker-Kleiner D, Cebotari S, Tudorache I, Suprunov M, Haverich A, and Hilfiker A: The pro-angiogenic factor CCN1 enhances the re-endothelialization of biological vascularized matrices in vitro. Cardiovasc Res 2010; 85, no. 4:806.

Cebotari S, Tudorache I, Jaekel T, Hilfiker A, Dorfman S, Ternes W, Haverich A, Lichtenberg A: Detergent decellularization of heart valves for tissue engineering: toxicological effects of residual detergents on human endothelial cells. Artif Organs 2010; 34, no. 3:206-10.

Hata H, Bär A, Dorfman S, Vukadinovic Z, Sawa Y, Haverich A, Hilfiker A: Engineering a novel three dimensional contractile myocardial patch with cell sheets and decellularised matrix. Eur J Cardiothorac Surg 2010; 38, no. 4:450.

Hess C, Wiegmann B, Maurer AN, Fischer P, Moller L, Martin U, Hilfiker A, Haverich A, and Fischer S: Reduced thrombocyte adhesion to endothelialized poly 4-methyl-1-pentene gas exchange membranes-a first step toward bioartificial lung development. Tissue Eng Part A 2010; 16, no. 10:3043.

Hilfiker-Kleiner D, Shukla P, Klein G, Schaefer A, Stapel B, Hoch M, Muller W, Scherr M, Theilmeier G, Ernst M, Hilfiker A, and Drexler H: Continuous glycoprotein-130-mediated signal transducer and activator of transcription-3 activation promotes inflammation, left ventricular rupture, and adverse outcome in subacute myocardial infarction. Circulation 2010; 122, no. 2:145.

Kühn C, Graf K, Heuer W, Hilfiker A, Chaberny IF, Stiesch M, and Haverich A: Economic implications of infections of implantable cardiac devices in a single institution. Eur J Cardiothorac Surg 2010; 37 no. 4:875.

Kühn C, Graf K, Mashaqi B, Pichlmaier M, Heuer W, Hilfiker A, Stiesch M, Chaberny IF, and Haverich A: Prevention of early vascular graft infection using regional antibiotic release. J Surg Res 2010; 164, no. 1:e185.

Shrestha M, Khaladj N, Baraki H, Al Ahmad A, Koigeldiyev N, Pichlmaier M, Haverich A, Hagl C: Aortic root reoperation: a technical challenge. J Heart Valve Dis. 2010; 19, no. 2:177-81.




Aper T, Haverich A, Teebken O: New developments in tissue engineering of vascular prosthetic grafts. Vasa 2009;38, no. 2:99-122.

Aper T, Simanowski J. Diagnostic and surgical treatment of the thrombophlebitis in the saphenofemoral junction: Ultraschall Med 2009;30, no. 2:180-184.

Baraki H, Tudorache I, Braun M, Hoffler K, Gorler A, Lichtenberg A, Bara C, Calistru A, Brandes G, Hewicker-Trautwein M, Hilfiker A, Haverich A, and Cebotari S: Orthotopic replacement of the aortic valve with decellularized allograft in a sheep model. Biomaterials 2009; 30, no. 31:6240.

Kallenbach K, Sorrentino S, Mertsching H, Kostin S, Pethig K, Haverich Md A, Cebotari S: A Novel Small Animal Model for Accelerated Investigation of Tissue Engineered Aortic Valved Conduits. Tissue Eng Part C Methods 2010;16, no. 1:41-50.

Kuehn C, Graf K, Heuer W, Hilfiker A, Chaberny IF, Stiesch M, Haverich A: Economic implications of infections of implantable cardiac devices in a single institution. Eur J Cardiothorac Surg 2009: DOI: 10.1016/j.ejcts.2009.10.018.

Shrestha M, Baraki H, Khaladj N, Koigeldiyev N, Haverich A, Hagl C: Do Patients Profit from repeat CABG? Open J Cardiovasc Surg 2009; no. 1:1-5.

Shrestha M, Baraki H, Khaladj N, Koigeldiyev N, Haverich A, Hagl C: Do Patients Profit from Third Time CABG? Open J Cardiovasc Surg 2009; no. 2:1-5.

Tudorache I, Kostin S, Meyer T, Teebken O, Bara C, Hilfiker A, Haverich A, Cebotari S: Viable vascularised autologous patch for transmural myocardial reconstruction. Eur J Cardiothorac Surg 2009;36, no. 2:306-11; discussion 311.

Aper T, Haverich A, Teebken OE: Der Traum vom idealen Bypassmaterial in der Gefäßchirurgie. Gefäßchirurgie 2008;13:87-98.

Shrestha M, Khaladj N, Baraki H, Haverich A, Hagl C: Is there an Age Limit for Cardiac Surgery? Clin Med: Geriatrics 2008:31-3.

Bär A, Haverich A, Hilfiker A: Cardiac tissue engineering: "reconstructing the motor of life". Scand J Surg 2007;96:154-8.

Hilfiker-Kleiner D, Kaminski K, Podewski E, Bonda T, Schaefer A, Sliwa K, Forster O, Quint A, Landmesser U, Doerries C, Luchtefeld M, Poli V, Schneider MD, Balligand JL, Desjardins F, Ansari A, Struman I, Nguyen NQ, Zschemisch NH, Klein G, Heusch G, Schulz R, Hilfiker A, Drexler H: A cathepsin D-cleaved 16 kDa form of prolactin mediates postpartum cardiomyopathy. Cell 2007;128:589-600.

Lichtenberg A, Cebotari S, Tudorache I, Hilfiker A, Haverich A: Biological scaffolds for heart valve tissue engineering. In: Hauser, Hansjörg; Fussenegger, Martin M Editors. Tissue Engineering, Series: Methods in Molecular Medicine. 2nd ed. New York: Humana Press; 2007, Vol. 140. p. 309-17.

Tudorache I, Cebotari S, Sturz G, Kirsch L, Hurschler C, Hilfiker A, Haverich A, Lichtenberg A: Tissue engineering of heart valves: biomechanical and morphological properties of decellularized heart valves. J Heart Valve Dis 2007;16:567-73; discussion 74.

Cebotari S, Lichtenberg A, Tudorache I, Hilfiker A, Mertsching H, Leyh R, Breymann T, Kallenbach K, Maniuc L, Batrinac A, Repin O, Maliga O, Ciubotaru A, and Haverich A: Clinical application of tissue engineered human heart valves using autologous progenitor cells. Circulation 2006;114, no. 1 Suppl:I132.

Giere B, Hilfiker A, Haverich A: Tissue engineering -- in vitro culture of reparative tissue. Dtsch Med Wochenschr 2006;131:860-2.

Hilfiker-Kleiner D, Hilfiker A, Castellazzi M, Wollert KC, Trautwein C, Schunkert H, Drexler H: JunD attenuates phenylephrine-mediated cardiomyocyte hypertrophy by negatively regulating AP-1 transcriptional activity. Cardiovasc Res 2006;71:108-17.

Lichtenberg A, Breymann T, Cebotari S, Haverich A: Cell seeded tissue engineered cardiac valves based on allograft and xenograft scaffolds. Progress in Pediatric Cardiology 2006;21:211-7.

Lichtenberg A, Cebotari S, Tudorache I, Sturz G, Winterhalter M, Hilfiker A, Haverich A: Flow-dependent re-endothelialization of tissue-engineered heart valves. J Heart Valve Dis 2006;15:287-93; discussion 93-4.

Lichtenberg A, Tudorache I, Cebotari S, Ringes-Lichtenberg S, Sturz G, Hoeffler K, Hurscheler C, Brandes G, Hilfiker A, Haverich A: In vitro re-endothelialization of detergent decellularized heart valves under simulated physiological dynamic conditions. Biomaterials 2006;27:4221-9.

Lichtenberg A, Tudorache I, Cebotari S, Suprunov M, Tudorache G, Goerler H, Park JK, Hilfiker-Kleiner D, Ringes-Lichtenberg S, Karck M, Brandes G, Hilfiker A, Haverich A: Preclinical testing of tissue-engineered heart valves re-endothelialized under simulated physiological conditions. Circulation 2006;114:I559-65.

Hilfiker-Kleiner D, Hilfiker A, and Drexler H: Many good reasons to have STAT3 in the heart. Pharmacol Ther 2005;107:131-7.

Schultheiss D, Gabouev AI, Cebotari S, Tudorache I, Walles T, Schlote N, Wefer J, Kaufmann PM, Haverich A, Jonas U, Stief CG, and Mertsching H: Biological vascularized matrix for bladder tissue engineering: matrix preparation, reseeding technique and short-term implantation in a porcine model. J Urol 2005;173:276-80.

Aper T, Puschmann C, Mertsching H, Haverich A, Teebken OE: Bioartifizielle Venenklappe. Biokompatibilität in einer Langzeitstudie im Schafmodell. Phlebologie 2004;33:160-165.

Aper T, Teebken OE, Steinhoff G, Haverich A: Use of a fibrin preparation in the engineering of a vascular graft model. Eur J Vasc Endovasc Surg 2004;28:296-302.

Baric D, Cebotari S, Mertsching H, Leyh R, Haverich A: Could 37 degrees C storage temperature extend homovital valve allograft viability? J Heart Valve Dis 2004;13:494-9; discussion 499-500.

Jagodzinski M, Cebotari S, Tudorache I, Zeichen J, Hankemeier S, Krettek C, van Griensven M, Mertsching H: Tissue engineering of long bones with a vascular matrix in a bioreactor. Orthopade, 2004;33(12):1394-1400

Kallenbach K, Leyh RG, Lefik E, Walles T, Wilhelmi M, Cebotari S, Schmiedl A, Haverich A, Mertsching H: Guided tissue regeneration: porcine matrix does not transmit PERV. Biomaterials 2004;25:3613-20.

Podewski EK, Hilfiker-Kleiner D, Hilfiker A, Morawietz H, Lichtenberg A, Wollert KC, Drexler H: Alterations in Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in patients with end-stage dilated cardiomyopathy. Circulation 2003;107:798-802.

Wollert KC, Podewski EK, Hilfiker-Kleiner D, Hilfiker A, Morawietz H, A. Lichtenberg A, Drexler H: What Are Appropriate Controls for Cardiac Interleukin-6 Expression in Heart Failure? Circulation. 2003;108(17):e129-30; author reply e129-30.

Hilfiker A, Hilfiker-Kleiner D, Fuchs M, Kaminski K, Lichtenberg A, Rothkötter HJ, Schieffer B, Drexler H: Expression of CYR61, an angiogenic immediate early gene, in arteriosclerosis and its regulation by angiotensin II. Circulation 2002;106:254-60.